Overview

  • Product nameAnti-MAP3K9 antibody - C-terminal
    See all MAP3K9 primary antibodies
  • Description
    Rabbit polyclonal to MAP3K9 - C-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Positive control
    • Human breast carcinoma tissue and rat testis tissue and A375 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa).

Target

  • FunctionActivates the JUN N-terminal pathway.
  • Tissue specificityExpressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
  • Information by UniProt
  • Database links
  • Alternative names
    • M3K9_HUMAN antibody
    • Map3k9 antibody
    • MEKK9 antibody
    • Mitogen activated protein kinase kinase kinase 9 antibody
    • Mitogen-activated protein kinase kinase kinase 9 antibody
    • Mixed lineage kinase 1 (tyr and ser/thr specificity) antibody
    • Mixed lineage kinase 1 antibody
    • MLK1 antibody
    • PRKE1 antibody
    see all

Anti-MAP3K9 antibody - C-terminal images

  • All lanes : Anti-MAP3K9 antibody - C-terminal (ab71628) at 1/100 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : rat testis tissue lysate

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 118 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • ab71628 at 1/50 dilution, staining MAP3K9 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-MAP3K9 antibody - C-terminal (ab71628)

This product has been referenced in:
  • Tivnan A  et al. MicroRNA-34a is a potent tumor suppressor molecule in vivo in neuroblastoma. BMC Cancer 11:33 (2011). WB ; Human . Read more (PubMed: 21266077) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"