Overview

  • Product nameAnti-MAP4 antibody
    See all MAP4 primary antibodies
  • Description
    Rabbit polyclonal to MAP4
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human MAP4.

    (Peptide available as ab99157.)

  • Positive control
    • This antibody gave a positive signal in human testis and spinal cord tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).

Target

  • FunctionNon-neuronal microtubule-associated protein. Promotes microtubule assembly.
  • Sequence similaritiesContains 4 Tau/MAP repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779A1753 antibody
    • MAP-4 antibody
    • MAP4 antibody
    • MAP4_HUMAN antibody
    • MGC8617 antibody
    • Microtubule associated protein 4 antibody
    • Microtubule-associated protein 4 antibody
    • OTTHUMP00000210723 antibody
    • OTTHUMP00000210725 antibody
    • OTTHUMP00000210727 antibody
    • OTTHUMP00000210730 antibody
    see all

Anti-MAP4 antibody images

  • MAP4 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MAP4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab89650.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 121kDa; MAP4

  • All lanes : Anti-MAP4 antibody (ab89650) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 121 kDa
    Observed band size : 121 kDa
    Additional bands at : 105 kDa (possible isoform).

    Exposure time : 8 minutes
  • ICC/IF image of ab89650 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89650 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HepG2 methanol fixed cells at 1ug/ml

References for Anti-MAP4 antibody (ab89650)

This product has been referenced in:
  • Courtheoux T  et al. Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase. Nat Commun 7:12810 (2016). ICC/IF ; Human . Read more (PubMed: 27641145) »
  • Zahnleiter D  et al. MAP4-dependent regulation of microtubule formation affects centrosome, cilia, and golgi architecture as a central mechanism in growth regulation. Hum Mutat 36:87-97 (2015). Read more (PubMed: 25323976) »

See all 2 Publications for this product

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Based on the immunogen sequence, this antibody will recongize the following isoformes listed on SwissProt (http://www.uniprot.org/uniprot/P27816): Isoform 1, 2, 4, 5, 6 and 7, however not isoform
3....

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (MDCK II (Madin-Darby canine kidney))
Specification MDCK II (Madin-Darby canine kidney)
Fixative Methanol
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2%
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Submitted May 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"