Overview

  • Product nameAnti-MAP4K1 antibody
    See all MAP4K1 primary antibodies
  • Description
    Rabbit polyclonal to MAP4K1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    A synthetic peptide corresponding to a region between amino acids 1 and 50 of human MAP4K1 (NP_009112.1).

  • Positive control
    • Jurkat whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 91 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
    • Tissue specificityExpressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CNH domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hematopoietic progenitor kinase 1 antibody
      • Hematopoietic progenitor kinase antibody
      • HPK1 antibody
      • Human Hematopoietic Progenitor Kinase antibody
      • M4K1_HUMAN antibody
      • Map4k1 antibody
      • MAPK/ERK kinase kinase kinase 1 antibody
      • MEK kinase kinase 1 antibody
      • MEKKK 1 antibody
      • Mitogen activated protein kinase kinase kinase kinase 1 antibody
      • Mitogen-activated protein kinase kinase kinase kinase 1 antibody
      see all

    Anti-MAP4K1 antibody images

    • All lanes : Anti-MAP4K1 antibody (ab86043) at 0.04 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : Jurkat whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 91 kDa
      Observed band size : 91 kDa


      Exposure time : 10 seconds
    • ab86043, at 0.4 µg/ml, staining MAP4K1 in Jurkat whole cell lysate immunoprecipitated using ab86043 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-MAP4K1 antibody (ab86043)

    ab86043 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"