WB, IHC-Pmore details Unsuitable for:
ICC or ICC/IF
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MAP4K1 aa 1-100 (N terminal).
WB: Raji and Daudi (human Burkitt's lymphoma) whole cell lysates.
IHC-P: Human liver tissue.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
ab33910 staining MAP4K1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
This product has been referenced in:
Li Y et al. Ubiquitin-specific protease 4 inhibits breast cancer cell growth through the upregulation of PDCD4. Int J Mol Med38:803-11 (2016).
Read more (PubMed: 27430936) »
Palmer RD et al. Pediatric malignant germ cell tumors show characteristic transcriptome profiles. Cancer Res68:4239-47 (2008).
Read more (PubMed: 18519683) »