Overview

  • Product nameAnti-MAP4K3 antibody
    See all MAP4K3 primary antibodies
  • Description
    Rabbit polyclonal to MAP4K3
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human MAP4K3.

  • Positive control
    • HepG2 cell line lysates. Brain tissue. This antibody gave a positive result in IF/ICC when used in the following methanol fixed cell lines: MALME-3M.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified through a protein A column, followed by protein affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 101 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceMay play a role in the response to environmental stress. This gene encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Germinal center kinase like kinase antibody
    • Germinal center kinase-related protein kinase antibody
    • GLK antibody
    • MAPK/ERK kinase kinase kinase 3 antibody
    • MAPKKKK3 antibody
    • MEK kinase kinase 3 antibody
    • MEKKK 3 antibody
    • MEKKK3 antibody
    • mitogen-activated protein kinase kinase kinase kinase 3 antibody
    • RAB8IPL1 antibody
    see all

Anti-MAP4K3 antibody images

  • Anti-MAP4K3 antibody (ab103481) at 1/100 dilution + HepG2 cell line lysates at 35 µg
    Developed using the ECL technique

    Predicted band size : 101 kDa
  • ab103481 at 1/50 dilution staining MAP4K3 in formalin-fixed and paraffin-embedded brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.
  • ICC/IF image of ab103481 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103481 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MAP4K3 antibody (ab103481)

This product has been referenced in:
  • Chu W  et al. Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B. Exp Ther Med 12:1741-1749 (2016). Read more (PubMed: 27602089) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"