Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP4K3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Mouse MAP4K3 aa 500-529 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q99JP0

  • Positive control
    • Mouse Neuro-2a cell line lysates; HepG2 cell line lysates; Neuro-2a cells and Mouse heart tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab173308 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 101 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    May play a role in the response to environmental stress. This gene encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Germinal center kinase like kinase antibody
    • Germinal center kinase-related protein kinase antibody
    • GLK antibody
    • MAPK/ERK kinase kinase kinase 3 antibody
    • MAPKKKK3 antibody
    • MEK kinase kinase 3 antibody
    • MEKKK 3 antibody
    • MEKKK3 antibody
    • mitogen-activated protein kinase kinase kinase kinase 3 antibody
    • RAB8IPL1 antibody
    see all

Images

  • Flow cytometric analysis of Neuro-2a cells (right histogram) labelling MAP4K3 at 1/10 dilution compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-MAP4K3 antibody (ab173308) at 1/100 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size: 101 kDa

  • Anti-MAP4K3 antibody (ab173308) at 1/100 dilution + Mouse Neuro-2a cell line lysates at 35 µg

    Predicted band size: 101 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse heart tissue labeling MAP4K3 with ab173308 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab173308 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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