The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionSerine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
Tissue specificityAppears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
HPK/GCK like kinase antibody
HPK/GCK like kinase HGK antibody
HPK/GCK-like kinase HGK antibody
MAPK/ERK kinase kinase kinase 4 antibody
MEK kinase kinase 4 antibody
MEKKK 4 antibody
Mitogen activated protein kinase kinase kinase kinase 4 antibody
Mitogen-activated protein kinase kinase kinase kinase 4 antibody
Nck interacting kinase antibody
Nck-interacting kinase antibody
Anti-MAP4K4 antibody images
Flow Cytometry - Anti-MAP4K4 antibody (ab56569)
Overlay histogram showing Jurkat cells stained with ab56569 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56569, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - MAP4K4 antibody (ab56569)
Western blot against tagged recombinant protein immunogen using ab56569 MAP4K4 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
References for Anti-MAP4K4 antibody (ab56569)
has not yet been referenced specifically in any publications.
Publishing research using ab56569? Please let us know so that we can cite the reference in this datasheet.
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