Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP4K4
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the internal region of human MAP4K4.

  • Positive control
    • A549 cells. Extracts from COLO and HUVEC cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 142 kDa (predicted molecular weight: 142 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
  • Tissue specificity
    Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLH21957 antibody
    • FLJ10410 antibody
    • FLJ20373 antibody
    • FLJ90111 antibody
    • Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
    • HGK antibody
    • HPK/GCK like kinase antibody
    • HPK/GCK like kinase HGK antibody
    • HPK/GCK-like kinase HGK antibody
    • KIAA0687 antibody
    • M4K4_HUMAN antibody
    • MAP4K4 antibody
    • MAPK/ERK kinase kinase kinase 4 antibody
    • MEK kinase kinase 4 antibody
    • MEKKK 4 antibody
    • MEKKK4 antibody
    • Mitogen activated protein kinase kinase kinase kinase 4 antibody
    • Mitogen-activated protein kinase kinase kinase kinase 4 antibody
    • Nck interacting kinase antibody
    • Nck-interacting kinase antibody
    • NIK antibody
    see all

Images

  • All lanes : Anti-MAP4K4 antibody (ab69885) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from HUVEC cells
    Lane 3 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 142 kDa
    Observed band size : 142 kDa
  • Immunofluorescence analysis of A549 cells using ab69885 at 1/500 dilution, in the presence or absence of the immunising peptide.

References

ab69885 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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