Overview

  • Product nameAnti-MAP4K4 antibody
    See all MAP4K4 primary antibodies
  • Description
    Rabbit polyclonal to MAP4K4
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 850 and 900 of human MAP4K4 (AAO32626.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab80418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 142 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
    • Tissue specificityAppears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CNH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLH21957 antibody
      • FLJ10410 antibody
      • FLJ20373 antibody
      • FLJ90111 antibody
      • Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
      • HGK antibody
      • HPK/GCK like kinase antibody
      • HPK/GCK like kinase HGK antibody
      • HPK/GCK-like kinase HGK antibody
      • KIAA0687 antibody
      • M4K4_HUMAN antibody
      • MAP4K4 antibody
      • MAPK/ERK kinase kinase kinase 4 antibody
      • MEK kinase kinase 4 antibody
      • MEKKK 4 antibody
      • MEKKK4 antibody
      • Mitogen activated protein kinase kinase kinase kinase 4 antibody
      • Mitogen-activated protein kinase kinase kinase kinase 4 antibody
      • Nck interacting kinase antibody
      • Nck-interacting kinase antibody
      • NIK antibody
      see all

    Anti-MAP4K4 antibody images

    • All lanes : Anti-MAP4K4 antibody (ab80418) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg

      Developed using the ECL technique

      Predicted band size : 142 kDa
      Observed band size : 171 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of Human MAP4K4 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80418 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80418 was used. Detection was by chemiluminescence with an exposure time of 30 seconds. Lane 2 represents control IgG IP.

    References for Anti-MAP4K4 antibody (ab80418)

    This product has been referenced in:
    • Santhana Kumar K  et al. The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma. Springerplus 4:19 (2015). WB ; Human . Read more (PubMed: 25625039) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab80418.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"