Human Predicted to work with:
Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human MAP4K4 aa 550-600. A region between residue 550 and 600 of human MAP4K4. Database link: AAO32626.1
Human Colon Carcinoma, Prostate Carcinoma
General notesThe concentration of IgG is less than or equal to 1 mg/ml. For purposes where you wish to run a normal rabbit IgG as a control, assuming 1 mg/ml for the concentration of IgG in the IHC antibody is reasonable.
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.
FunctionSerine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
Tissue specificityAppears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.