Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP4K5
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 417-482 (HCPDSESRAP QILRRQSSPS CGPVAETSSI GNGDGISKLM SENTEGSAQA PQLPRKNDKR DFPKPA) of Human MAP4K5 (NP_942089).

  • Positive control
    • MOLT4 whole cell lysate; A431 cells; SAS xenograft; 293T, H1299, HepG2 and Raji cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab96551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 95 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Relevance
    MAP4K5, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress. It appears to act upstream of the JUN N-terminal pathway. MAP4K5 interacts with both SH3 domains of the adapter proteins CRK and CRKL. This cytoplasmic protein is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • KHS antibody
    • Kinase homologous to SPS1/STE20 antibody
    • MAPK/ERK kinase kinase kinase 5 antibody
    • MEK kinase kinase 5 antibody
    • MEKKK 5 antibody
    see all

Images

  • Anti-MAP4K5 antibody (ab96551) at 1/5000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 95 kDa
  • ab96551, at 1/200 dilution, staining MAP4K5 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is merged with DNA probe.
  • ab96551, at 1/100 dilution, staining MAP4K5 in paraffin-embedded SAS xenograft by Immunohistochemistry.

References

ab96551 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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