RelevanceMAP4K6 is a serine/threonine kinase belonging to the germinal center kinase (GCK) family. These kinases regulate a wide variety of cellular processes by coupling cell surface receptors to MAPK pathways. This kinase appears to act upstream of the JUN N-terminal pathway and may be involved in brain development. It may also play a role in the response to environmental stress, in cytoskeleton reorganization, cell adhesion, and cell motility. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified.
Detection of MAP4K6 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86385 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ICC/IF image of ab86385 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86385, 5µg/ml) overnight at +4°C. The secondary antibody (green) was goat anti-rabbit DyLight 488 (IgG H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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