Overview

  • Product nameAnti-MAP4K6 antibody
    See all MAP4K6 primary antibodies
  • Description
    Rabbit polyclonal to MAP4K6
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 775 and 825 of Human MAP4K6 (NP_722549.2).

  • Positive control
    • Whole cell lysates from HeLa and mouse NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab86385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 150 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • RelevanceMAP4K6 is a serine/threonine kinase belonging to the germinal center kinase (GCK) family. These kinases regulate a wide variety of cellular processes by coupling cell surface receptors to MAPK pathways. This kinase appears to act upstream of the JUN N-terminal pathway and may be involved in brain development. It may also play a role in the response to environmental stress, in cytoskeleton reorganization, cell adhesion, and cell motility. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified.
  • Cellular localizationCytoplasm. Cell junction; synapse; postsynaptic cell membrane; postsynaptic density. Cell projection; axon. Cell projection; dendrite. Isoform 4: Golgi apparatus
  • Database links
  • Alternative names
    • B55 antibody
    • GCK family kinase MiNK antibody
    • hMINK antibody
    • Map4k6 antibody
    • MAPK/ERK kinase kinase kinase 6 antibody
    • MEK (MAP/ERK) kinase kinase 6 antibody
    • MEK kinase kinase 6 antibody
    • MEKKK6 antibody
    • MGC21111 antibody
    • MINK 1 antibody
    • MINK antibody
    • MINK1 antibody
    • Misshapen like kinase 1 antibody
    • Misshapen NIKs Related kinase antibody
    • Misshapen/NIK related kinase antibody
    • Mitogen activated protein kinase kinase kinase kinase 6 antibody
    • RP23-122P1.6 antibody
    • Ysk2 antibody
    • ZC3 antibody
    see all

Anti-MAP4K6 antibody images

  • All lanes : Anti-MAP4K6 antibody (ab86385) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 150 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Detection of MAP4K6 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86385 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • ICC/IF image of ab86385 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86385, 5µg/ml) overnight at +4°C. The secondary antibody (green) was goat anti-rabbit DyLight 488 (IgG H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MAP4K6 antibody (ab86385)

ab86385 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification Hippocampal homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"