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Rabbit polyclonal to MAP4K6 - C-terminal
Recombinant fragment, corresponding to a region within amino acids 901-1120 of Human MAP4K6 (Uniprot ID: Q8N4C8).
HepG2 whole cell lysate; U87 xenograft; HeLa cells
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 150 kDa.
1/100 - 1/1000.
1/100 - 1/1000.
MAP4K6 is a serine/threonine kinase belonging to the germinal center kinase (GCK) family. These kinases regulate a wide variety of cellular processes by coupling cell surface receptors to MAPK pathways. This kinase appears to act upstream of the JUN N-terminal pathway and may be involved in brain development. It may also play a role in the response to environmental stress, in cytoskeleton reorganization, cell adhesion, and cell motility. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified.
Cytoplasm. Cell junction; synapse; postsynaptic cell membrane; postsynaptic density. Cell projection; axon. Cell projection; dendrite.
Isoform 4: Golgi apparatus
GCK family kinase MiNK antibody
Western blot - Anti-MAP4K6 antibody - C-terminal (ab154256)
Anti-MAP4K6 antibody - C-terminal (ab154256) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 150 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K6 antibody - C-terminal (ab154256)
Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling MAP4K6 with ab154256 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MAP4K6 antibody - C-terminal (ab154256)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAP4K6 with ab154256 at 1/200 dilution. Lower panel merged with DNA probe.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"