Overview

  • Product name
    Anti-MAP7D3 antibody
  • Description
    Rabbit polyclonal to MAP7D3
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: EADNEESDKD SLNEMFPSAI LNGTGSPTKF KMPFNNAKKM THKLVFLEDG TSQVRKEPKT YFNGDLKNFR QKSMKDTSIQ EVVSRPSSKR MTSH, corresponding to amino acids 745-838 of Human MAP7D3 (Q8IWC1).

  • Positive control
    • Human cell line A-431, Human Placenta.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 60% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

Images

  • ab122286, at 1/2000 dilution, staining Human placenta trophoblastic cells by immunohistochemistry of paraffin embedded tissues (IHC-P).
  • ab122286, at 4 µg/mL, staining Human cell line A-431 by immunofluorescence (IF). Antibody staining is shown in green.

References

This product has been referenced in:
  • Yadav S  et al. C-terminal region of MAP7 domain containing protein 3 (MAP7D3) promotes microtubule polymerization by binding at the C-terminal tail of tubulin. PLoS One 9:e99539 (2014). Read more (PubMed: 24927501) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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