Overview

  • Product nameAnti-MAPK15 antibody
    See all MAPK15 primary antibodies
  • Description
    Mouse polyclonal to MAPK15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length MAPK15 protein (Human)

  • Positive control
    • transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 60 kDa).

Target

  • FunctionIn vitro, phosphorylates MBP.
  • Tissue specificityWidely expressed with a maximal expression in lung and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
    Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK-7 antibody
    • ERK-8 antibody
    • ERK7 antibody
    • ERK8 antibody
    • Extracellular regulated kinase 8 delta antibody
    • Extracellular signal regulated kinase 8 antibody
    • Extracellular signal-regulated kinase 7 antibody
    • Extracellular signal-regulated kinase 8 antibody
    • MAP kinase 15 antibody
    • MAPK 15 antibody
    • Mapk15 antibody
    • Mitogen activated protein kinase 15 antibody
    • Mitogen-activated protein kinase 15 antibody
    • MK15_HUMAN antibody
    see all

Anti-MAPK15 antibody images

  • All lanes : Anti-MAPK15 antibody (ab67086) at 1/500 dilution

    Lane 1 : MAPK15 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 60 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-MAPK15 antibody (ab67086)

ab67086 has not yet been referenced specifically in any publications.

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