The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionIn vitro, phosphorylates MBP.
Tissue specificityWidely expressed with a maximal expression in lung and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
DomainThe N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation. The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modificationsDually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
Predicted band size : 60 kDa Western blot analysis of untransfected HEK-293T (lane 1) and MAPK15 transfected HEK-293T (lane 2) lysates (50µg/lane) labeling MAPK15 with ab86275 at 1:2000. A HRP-conjugtaed anti-rabbit (1:1000) was used as the secondary antibody.