Anti-MAPK15 antibody - N-terminal (ab70911)

Overview

  • Product name
    Anti-MAPK15 antibody - N-terminal
    See all MAPK15 primary antibodies
  • Description
    Rabbit polyclonal to MAPK15 - N-terminal
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N-terminal region of human MAPK15

  • Positive control
    • T47D cell lysate; Paraffin embedded Human breast carcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 60 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Function
    In vitro, phosphorylates MBP.
  • Tissue specificity
    Widely expressed with a maximal expression in lung and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
    Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK-7 antibody
    • ERK-8 antibody
    • ERK7 antibody
    • ERK8 antibody
    • Extracellular regulated kinase 8 delta antibody
    • Extracellular signal regulated kinase 8 antibody
    • Extracellular signal-regulated kinase 7 antibody
    • Extracellular signal-regulated kinase 8 antibody
    • MAP kinase 15 antibody
    • MAPK 15 antibody
    • Mapk15 antibody
    • Mitogen activated protein kinase 15 antibody
    • Mitogen-activated protein kinase 15 antibody
    • MK15_HUMAN antibody
    see all

Images

  • All lanes : Anti-MAPK15 antibody - N-terminal (ab70911) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell line lysate
    Lane 2 : 293 cell line lysate transiently transfected with the MAPK15 gene

    Lysates/proteins at 2 µg per lane.


    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Anti-MAPK15 antibody - N-terminal (ab70911) at 1/60 dilution + T47D cell line lysates at 35 µg

    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab70911, at a 1/10 dilution, staining human MAPK15 in breast carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70911 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References

ab70911 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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