Overview

  • Product nameAnti-MAPK4 antibody
    See all MAPK4 primary antibodies
  • Description
    Rabbit polyclonal to MAPK4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide from within internal sequence amino acids 288-337: FLEKILTFNP MDRLTAEMGL QHPYMSPYSC PEDEPTSQHP FRIEDEIDDI, of Human MAPK4 (NP_002738)

  • Positive control
    • Human fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAtypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.
  • Tissue specificityHigh expression in heart and brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe FRIEDE motif is required for docking MAPKAPK5.
    In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
  • Cellular localizationCytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK-4 antibody
    • ERK3 antibody
    • Erk3 related antibody
    • ERK4 antibody
    • Extracellular signal regulated kinase 4 antibody
    • Extracellular signal-regulated kinase 4 antibody
    • MAP kinase 4 antibody
    • MAP kinase isoform p63 antibody
    • MAPK 4 antibody
    • MAPK4 antibody
    • Mitogen activated protein kinase 4 antibody
    • Mitogen-activated protein kinase 4 antibody
    • MK04_HUMAN antibody
    • p63 MAPK antibody
    • p63-MAPK antibody
    • p63MAPK antibody
    • PRKM4 antibody
    • Protein kinase, mitogen-activated, 4 antibody
    • Protein-tyrosine kinase ERK3-related antibody
    • RMNK2 antibody
    see all

Anti-MAPK4 antibody images

  • Anti-MAPK4 antibody (ab94617) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa

References for Anti-MAPK4 antibody (ab94617)

ab94617 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"