Overview

  • Product nameAnti-MAPK4 antibody
    See all MAPK4 primary antibodies
  • Description
    Rabbit polyclonal to MAPK4
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 296 of MAPK4 (NP_002738).

  • Positive control
    • WB: H1299 whole cell lysate; A431, HelaS3, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab96816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionAtypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.
  • Tissue specificityHigh expression in heart and brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe FRIEDE motif is required for docking MAPKAPK5.
    In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
  • Cellular localizationCytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK-4 antibody
    • ERK3 antibody
    • Erk3 related antibody
    • ERK4 antibody
    • Extracellular signal regulated kinase 4 antibody
    • Extracellular signal-regulated kinase 4 antibody
    • MAP kinase 4 antibody
    • MAP kinase isoform p63 antibody
    • MAPK 4 antibody
    • MAPK4 antibody
    • Mitogen activated protein kinase 4 antibody
    • Mitogen-activated protein kinase 4 antibody
    • MK04_HUMAN antibody
    • p63 MAPK antibody
    • p63-MAPK antibody
    • p63MAPK antibody
    • PRKM4 antibody
    • Protein kinase, mitogen-activated, 4 antibody
    • Protein-tyrosine kinase ERK3-related antibody
    • RMNK2 antibody
    see all

Anti-MAPK4 antibody images

  • Anti-MAPK4 antibody (ab96816) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 66 kDa
  • Immunofluorescence analysis of MAPK4 in paraformaldehyde fixed HeLa, using ab96816 at a 1/200 dilution.
    The lower image was merged with DNA probe.

References for Anti-MAPK4 antibody (ab96816)

ab96816 has not yet been referenced specifically in any publications.

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