Overview

  • Product name
    Anti-MAPK6 antibody [EP1720Y]
    See all MAPK6 primary antibodies
  • Description
    Rabbit monoclonal [EP1720Y] to MAPK6
  • Tested applications
    Suitable for: IP, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MAPK6 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q16659

  • Positive control
    • HeLa cell lysate or human breast carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab53277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 22505454
WB 1/1000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 63 kDa).
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
    • Tissue specificity
      Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications
      Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.
    • Information by UniProt
    • Database links
    • Alternative names
      • ERK-3 antibody
      • ERK3 antibody
      • Extracellular signal regulated kinase 3 antibody
      • Extracellular signal regulated kinase p97 antibody
      • Extracellular signal-regulated kinase 3 antibody
      • HsT17250 antibody
      • MAP kinase 6 antibody
      • MAP kinase isoform p97 antibody
      • MAPK 6 antibody
      • MAPK6 antibody
      • Mitogen activated protein kinase 6 antibody
      • Mitogen-activated protein kinase 6 antibody
      • MK06_HUMAN antibody
      • p97 MAPK antibody
      • p97-MAPK antibody
      • PRKM6 antibody
      • Protein kinase mitogen activated 5 antibody
      • Protein kinase mitogen activated 6 antibody
      see all

    Images

    • Anti-MAPK6 antibody [EP1720Y] (ab53277) at 1/1000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 63 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing A431 cells stained with ab53277 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53277, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Ab53277 (1:50) staining human MAPK6 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

    References

    This product has been referenced in:
    • Ling S  et al. Metformin potentiates the effect of arsenic trioxide suppressing intrahepatic cholangiocarcinoma: roles of p38 MAPK, ERK3, and mTORC1. J Hematol Oncol 10:59 (2017). Read more (PubMed: 28241849) »
    • Mathien S  et al. Deubiquitinating Enzyme USP20 Regulates Extracellular Signal-Regulated Kinase 3 Stability and Biological Activity. Mol Cell Biol 37:N/A (2017). IP ; Human . Read more (PubMed: 28167606) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab53277.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up