Overview

  • Product name
    Anti-MAPK6 (phospho S189) antibody
    See all MAPK6 primary antibodies
  • Description
    Rabbit polyclonal to MAPK6 (phospho S189)
  • Specificity
    ab74032 detects endogenous levels of MAPK6 only when phosphorylated at serine 189.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human MAPK6 around the phosphorylation site of serine 189 (H-L-SP-E-G). (SwissProt Q16659.)

  • Positive control
    • Human breast carcinoma tissue. Extracts from mouse brain.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab74032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
  • Tissue specificity
    Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK-3 antibody
    • ERK3 antibody
    • Extracellular signal regulated kinase 3 antibody
    • Extracellular signal regulated kinase p97 antibody
    • Extracellular signal-regulated kinase 3 antibody
    • HsT17250 antibody
    • MAP kinase 6 antibody
    • MAP kinase isoform p97 antibody
    • MAPK 6 antibody
    • MAPK6 antibody
    • Mitogen activated protein kinase 6 antibody
    • Mitogen-activated protein kinase 6 antibody
    • MK06_HUMAN antibody
    • p97 MAPK antibody
    • p97-MAPK antibody
    • PRKM6 antibody
    • Protein kinase mitogen activated 5 antibody
    • Protein kinase mitogen activated 6 antibody
    see all

Images

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab74032, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising phosphopeptide.
  • All lanes : Anti-MAPK6 (phospho S189) antibody (ab74032) at 1/500 dilution

    Lane 1 : Extracts from mouse brain
    Lane 2 : Extracts from mouse brain with immunising phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Ling S  et al. Metformin potentiates the effect of arsenic trioxide suppressing intrahepatic cholangiocarcinoma: roles of p38 MAPK, ERK3, and mTORC1. J Hematol Oncol 10:59 (2017). Read more (PubMed: 28241849) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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