Validated using a knockout cell line
Recombinant
RabMAb

Anti-MAPKAP Kinase 2 antibody [E341] (ab32567)

Overview

  • Product name
    Anti-MAPKAP Kinase 2 antibody [E341]
    See all MAPKAP Kinase 2 primary antibodies
  • Description
    Rabbit monoclonal [E341] to MAPKAP Kinase 2
  • Specificity
    ab32567 recognises MAPKAP Kinase-2.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near C-terminus of human MAPKKAP Kinase-2.

  • Positive control
    • Hela cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IP 1/200.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC.
  • Target

    • Function
      Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
    • Tissue specificity
      Expressed in all tissues examined.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated and activated by MAP kinase.
    • Information by UniProt
    • Database links
    • Alternative names
      • MAP kinase activated protein Kinase 2 antibody
      • MAP kinase-activated protein kinase 2 antibody
      • MAPK activated protein kinase 2 antibody
      • MAPK-activated protein kinase 2 antibody
      • MAPK2_HUMAN antibody
      • MAPKAP K2 antibody
      • MAPKAP kinase 2 antibody
      • MAPKAPK 2 antibody
      • MAPKAPK-2 antibody
      • MAPKAPK2 antibody
      • Mitogen activated protein kinase activated protein kinase 2 antibody
      • MK 2 antibody
      • MK2 antibody
      see all

    Images



    • Predicted band size : 46 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Lanes 1 - 2 : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
      Lanes 3 - 4 : Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/500 dilution

      Lane 1 : UV treated HeLa cells
      Lane 2 : UV treated HeLa cells + p38 inhibitor
      Lane 3 : UV treated HeLa cells
      Lane 4 : UV treated HeLa cells + p38 inhibitor

      Secondary
      IRDye® 680 LICOR goat anti-rabbit.

      Performed under reducing conditions.

      Predicted band size : 46 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible cross reactivity).

      Exposure time : 1 minuteab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).

      See Abreview

    References

    ab32567 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    35 µg
    Specification
    HeLa cells
    Treatment
    UV treated samples (20 J dose)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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