Overview

  • Product name
    Anti-MAPKAP Kinase 3 antibody
    See all MAPKAP Kinase 3 primary antibodies
  • Description
    Rabbit polyclonal to MAPKAP Kinase 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 89-382 of Human MAPKAP Kinase 3 (Uniprot ID: Q16644).

  • Positive control
    • H1299, HeLaS3 and Mouse spleen lysates; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
  • Tissue specificity
    Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3pK antibody
    • anti MAPKAPK3 antibody
    • Chromosome 3p kinase antibody
    • EC 2.7.11.1 antibody
    • MAP kinase-activated protein kinase 3 antibody
    • MAPK activated protein kinase 3 antibody
    • MAPK-activated protein kinase 3 antibody
    • MAPK3_HUMAN antibody
    • MAPKAP antibody
    • MAPKAP kinase 3 antibody
    • MAPKAP-K3 antibody
    • MAPKAP3 antibody
    • MAPKAPK 3 antibody
    • MAPKAPK-3 antibody
    • Mapkapk3 antibody
    • MAPKAPK3 antibody antibody
    • Mitogen activated protein kinase activated protein kinase 3 antibody
    • Mitogen activated protein kinase activated protein kinase antibody
    • MK-3 antibody
    see all

Images

  • All lanes : Anti-MAPKAP Kinase 3 antibody (ab155178) at 1/3000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 43 kDa



    10% SDS PAGE
  • Anti-MAPKAP Kinase 3 antibody (ab155178) at 1/10000 dilution + Mouse spleen whole cell lysate at 50 µg

    Predicted band size: 43 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MAPKAP Kinase 3 with ab155178 at 1/200 dilution (green). Lower image shows merge with DNA probe (blue).

References

ab155178 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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