Overview

  • Product nameAnti-MAPKAP Kinase 3 antibody
    See all MAPKAP Kinase 3 primary antibodies
  • Description
    Mouse monoclonal to MAPKAP Kinase 3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 272-382 of Human MAPKAP Kinase 3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionStress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
    • Tissue specificityWidely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313.
    • Cellular localizationNucleus. Cytoplasm. Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3pK antibody
      • anti MAPKAPK3 antibody
      • Chromosome 3p kinase antibody
      • EC 2.7.11.1 antibody
      • MAP kinase-activated protein kinase 3 antibody
      • MAPK activated protein kinase 3 antibody
      • MAPK-activated protein kinase 3 antibody
      • MAPK3_HUMAN antibody
      • MAPKAP antibody
      • MAPKAP kinase 3 antibody
      • MAPKAP-K3 antibody
      • MAPKAP3 antibody
      • MAPKAPK 3 antibody
      • MAPKAPK-3 antibody
      • Mapkapk3 antibody
      • MAPKAPK3 antibody antibody
      • Mitogen activated protein kinase activated protein kinase 3 antibody
      • Mitogen activated protein kinase activated protein kinase antibody
      • MK-3 antibody
      see all

    Anti-MAPKAP Kinase 3 antibody images

    • MAPKAP Kinase 3 antibody (ab58189) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.


    • Predicted band size : 43 kDa
      MAPKAP Kinase 3 antibody (ab58189) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-MAPKAP Kinase 3 antibody (ab58189)

    ab58189 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab58189.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"