Anti-MAPKAP Kinase 3 antibody - N-terminal (ab172878)


  • Product name
    Anti-MAPKAP Kinase 3 antibody - N-terminal
    See all MAPKAP Kinase 3 primary antibodies
  • Description
    Rabbit polyclonal to MAPKAP Kinase 3 - N-terminal
  • Host species
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide within Mouse MAPKAP Kinase 3 aa 62-90 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q3UMW7

  • Positive control
    • K562 cell lysate; Mouse stomach tissue lysate; Mouse skeletal muscle tissue.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab172878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.


  • Function
    Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
  • Tissue specificity
    Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3pK antibody
    • anti MAPKAPK3 antibody
    • Chromosome 3p kinase antibody
    • EC antibody
    • MAP kinase-activated protein kinase 3 antibody
    • MAPK activated protein kinase 3 antibody
    • MAPK-activated protein kinase 3 antibody
    • MAPK3_HUMAN antibody
    • MAPKAP antibody
    • MAPKAP kinase 3 antibody
    • MAPKAP-K3 antibody
    • MAPKAP3 antibody
    • MAPKAPK 3 antibody
    • MAPKAPK-3 antibody
    • Mapkapk3 antibody
    • MAPKAPK3 antibody antibody
    • Mitogen activated protein kinase activated protein kinase 3 antibody
    • Mitogen activated protein kinase activated protein kinase antibody
    • MK-3 antibody
    see all


  • Anti-MAPKAP Kinase 3 antibody - N-terminal (ab172878) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 43 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse skeletal muscle tissue labeling MAPKAP Kinase 3 with ab172878 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-MAPKAP Kinase 3 antibody - N-terminal (ab172878) at 1/100 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size: 43 kDa


ab172878 has not yet been referenced specifically in any publications.

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