Overview

  • Product nameAnti-MAPKAPK2/3 antibody
  • Description
    Rabbit polyclonal to MAPKAPK2/3
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 332-336 of Human MAPKAPK2

  • Positive control
    • Recombinant proteins

Properties

Applications

Our Abpromise guarantee covers the use of ab82543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 43 , 45 kDa.

Target

  • RelevanceMAPKAPK2 (MAP kinase activated protein kinase 2, MAPKAP Kinase 2), also known as p45 hsp27 kinase, is a 45-54 kDa serine/threonine protein kinase that contains a proline rich sequence and two putative SH3 binding sites. MAPKAP Kinase 2 is activated in response to stress, IL1 and TNF, possibly catalyzed by p38/Hog dependent phosphorylation. One of the major substrates of MAPKAP Kinase 2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. MAPKAP2 is implicated in several disorders including ischemic brain injury and heart failure and has been shown to be important in regulating stress resistance and the production of TNF alpha. MAPKAPK3 functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • 3PK antibody
    • AA960234 antibody
    • MAP kinase activated protein kinase 2 antibody
    • MAP kinase activated protein kinase 3 antibody
    • MAPK activated protein kinase 2 antibody
    • MAPK activated protein kinase 3 antibody
    • MAPKAP kinase 2 antibody
    • MAPKAP kinase 3 antibody
    • MK2 antibody
    • MK3 antibody
    • OTTHUMP00000034531 antibody
    • Rps6kc1 antibody
    see all

Anti-MAPKAPK2/3 antibody images

  • All lanes : Anti-MAPKAPK2/3 antibody (ab82543) at 1/1000 dilution

    Lane 1 : Human recombinant MAPKAPK2 with proprietary tag
    Lane 2 : human recombinant MAPKAPK3 with proprietary tag

    Lysates/proteins at 0.02 µg per lane.


    Predicted band size : 43 , 45 kDa
    Observed band size : 65,70 kDa (why is the actual band size different from the predicted?)

References for Anti-MAPKAPK2/3 antibody (ab82543)

ab82543 has not yet been referenced specifically in any publications.

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