Overview

  • Product nameAnti-MAPRE1 antibody
    See all MAPRE1 primary antibodies
  • Description
    Rabbit polyclonal to MAPRE1
  • Specificityab50188 recognizes EB1. The immunizing sequence has limited homology to human EB2 (35% identity) and human EB3 (53% identity).
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    GFVIPDEGGPQEEQEEY

    conjugated to KLH, corresponding to C terminal amino acids 252-268 of Human MAPRE1

  • Positive control
    • Cell lysate of the rat fibroblast cell line Rat1, Rat1 cells and a HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab50188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notesICC/IF: Use at a concentration of 1 - 2 µg/ml.
    IP: 10-20 ug of the antibody can immunoprecipitate EB1 from a cell lysate of HeLa cells.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa. Staining of the EB1 band in immunoblotting is specifically inhibited with the immunizing peptide.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
    • Tissue specificityUbiquitously expressed.
    • Sequence similaritiesBelongs to the MAPRE family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 EB1 C-terminal domain.
    • DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
    • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5530600P05Rik antibody
      • Adenomatous polyposis coli binding protein EB 1 antibody
      • Adenomatous polyposis coli binding protein EB1 antibody
      • AI462499 antibody
      • AI504412 antibody
      • APC binding protein EB 1 antibody
      • APC binding protein EB1 antibody
      • APC-binding protein EB1 antibody
      • AW260097 antibody
      • BIM1p antibody
      • D2Ertd459e antibody
      • EB 1 antibody
      • EB1 antibody
      • End binding protein 1 antibody
      • End-binding protein 1 antibody
      • fa01e12 antibody
      • fc23e11 antibody
      • fi33c06 antibody
      • MAPRE 1 antibody
      • MAPRE1 antibody
      • Mapre3 antibody
      • MARE1_HUMAN antibody
      • MGC117374 antibody
      • MGC129946 antibody
      • MGC52508 antibody
      • Microtubule associated protein RP/EB family member 1 antibody
      • Microtubule-associated protein RP/EB family member 1 antibody
      • wu:fa01e12 antibody
      • wu:fc23e11 antibody
      • wu:fi33c06 antibody
      • zgc:55428 antibody
      • zgc:77807 antibody
      • zgc:85755 antibody
      see all

    Anti-MAPRE1 antibody images

    • ab50188 at 1ug/ml dilution staining the “plus-end tracking protein” (+TIP) structures in Rat1 fibroblasts grown to semi-confluency and fixed with acetone/methanol. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. Antibody dilution 1:1,000.
    • All lanes : Anti-MAPRE1 antibody (ab50188) at 0.5 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : NIH3T3 cell lysate
      Lane 3 : Rat1 cell lysate
      Lane 4 : A431 cell lysate
      Lane 5 : SW48 cell lysate
      Lane 6 : HCT116 cell lysate
      Lane 7 : HEK293 cell lysate
      Lane 8 : HeLa cell lysate with EB1 immunizing peptide at 10 µg/ml

      Secondary
      Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution

      Predicted band size : 30 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    • Lane 1 : No primary antibody
      Lanes 2 - 4 : Anti-MAPRE1 antibody (ab50188) at 15 µg

      Lane 1 : No lysate
      Lane 2 : No lysate
      Lane 3 : HeLa cell lysate at 100 µg
      Lane 4 : HeLa cell lysate at 25 µg

      Secondary
      Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution

      Predicted band size : 30 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-MAPRE1 antibody (ab50188)

    This product has been referenced in:
    • Shimozawa T  et al. Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging. Proc Natl Acad Sci U S A 110:3399-404 (2013). ICC/IF ; Human . Read more (PubMed: 23401517) »
    • Goldspink DA  et al. The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation. J Cell Sci 126:4000-14 (2013). Read more (PubMed: 23813963) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Dog Cell (MDCK cysts)
    Permeabilization Yes - Tritón 0.3 %
    Specification MDCK cysts
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 16°C
    Fixative Methanol
    Username

    Dr. M. Cecilia Larocca

    Verified customer

    Submitted Jan 28 2016

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Mouse Cell (NIH 3T3)
    Specification NIH 3T3
    Permeabilization Yes - Triton X-100
    Fixative Glutaraldehyde + Formaldehyde
    Username

    Mr. Ian Kent

    Verified customer

    Submitted Dec 12 2014

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization No
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted May 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"