The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
Use at 10 µg/mg of lysate.
FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the MAPRE family. Contains 1 CH (calponin-homology) domain. Contains 1 EB1 C-terminal domain.
DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
Adenomatous polyposis coli binding protein EB 1 antibody
Adenomatous polyposis coli binding protein EB1 antibody
APC binding protein EB 1 antibody
APC binding protein EB1 antibody
APC-binding protein EB1 antibody
EB 1 antibody
End binding protein 1 antibody
End-binding protein 1 antibody
MAPRE 1 antibody
Microtubule associated protein RP/EB family member 1 antibody
Microtubule-associated protein RP/EB family member 1 antibody
Anti-MAPRE1 antibody images
Western blot - MAPRE1 antibody (ab86598)
All lanes : Anti-MAPRE1 antibody (ab86598) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Immunoprecipitation/ Western Blot of ab86598
Lane 1: ab86598 at 10µg/mg whole cell lysate.
Lane 2: Control IgG.
ab86598 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
References for Anti-MAPRE1 antibody (ab86598)
has not yet been referenced specifically in any publications.
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