Overview

  • Product nameAnti-MARCH-I antibody
    See all MARCH-I primary antibodies
  • Description
    Rabbit polyclonal to MARCH-I
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (LQASSPTTGT APRSQSRLSV CPSTQDICRI CHCEGDEESP LITPCRCTGT) of human MARCH-I (NP_060393).

  • Positive control
    • Fetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, downregulates their cell surface localization thus sequestering them in the intracellular endosomal system.
  • Tissue specificityExpressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B cells, moderate in dendritic cells and low in splenic T cells.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 RING-CH-type zinc finger.
  • Developmental stageDuring maturation of dendritic cells, expression is down-regulated and stabilizes MHC class II proteins accumulate at the plasma membrane.
  • DomainThe RING-CH-type zinc finger domain is required for E3 ligase activity.
  • Post-translational
    modifications
    Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins.
  • Cellular localizationGolgi apparatus > trans-Golgi network membrane. Lysosome membrane. Cytoplasmic vesicle membrane. Late endosome membrane. Early endosome membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp564M1682 antibody
    • E3 ubiquitin protein ligase MARCH1 antibody
    • E3 ubiquitin-protein ligase MARCH1 antibody
    • FLJ20668 antibody
    • MARCH 1 antibody
    • MARCH-I antibody
    • March1 antibody
    • MARH1_HUMAN antibody
    • Membrane associated RING CH protein I antibody
    • Membrane associated ring finger (C3HC4) 1 antibody
    • Membrane associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase antibody
    • Membrane associated RING finger protein 1 antibody
    • Membrane-associated RING finger protein 1 antibody
    • Membrane-associated RING-CH protein I antibody
    • RING finger protein 171 antibody
    • RNF171 antibody
    see all

Anti-MARCH-I antibody images

  • Anti-MARCH-I antibody (ab83469) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

References for Anti-MARCH-I antibody (ab83469)

ab83469 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"