Anti-MARCH3 antibody (ab137261)
Key features and details
- Rabbit polyclonal to MARCH3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-MARCH3 antibody -
Description
Rabbit polyclonal to MARCH3 -
Host species
Rabbit -
Specificity
ab137261 detects endogenous levels of total MARCH3 protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human MARCH3 aa 111-160 (internal sequence).
Sequence:SYCELCHFRFAVERKPRPLV EWLRNPGPQH EKRTLFGDMV CFLFITPLAT
Database link: Q86UD3 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab137261 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-CH-type zinc finger. -
Domain
The RING-CH-type zinc finger domain is required for E3 ligase activity. -
Cellular localization
Cytoplasmic vesicle membrane. Early endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 115123 Human
- Entrez Gene: 320253 Mouse
- Omim: 613333 Human
- SwissProt: Q86UD3 Human
- SwissProt: Q8BRX9 Mouse
- Unigene: 132441 Human
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Alternative names
- E3 ubiquitin protein ligase MARCH3 antibody
- E3 ubiquitin-protein ligase MARCH3 antibody
- FLJ20096 antibody
see all
Images
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Anti-MARCH3 antibody (ab137261) at 1/500 dilution + Raw264.7 cell lysate at 30 µg
Predicted band size: 29 kDa -
All lanes : Anti-MARCH3 antibody (ab137261) at 1/500 dilution
Lane 1 : K562 cell line
Lane 2 : COS7 cell line
Lane 3 : K562 cell line with Blocking with synthesized peptide
Predicted band size: 29 kDa -
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using ab137261 at 1:50 dilution. The picture on the right is blocked with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137261 has not yet been referenced specifically in any publications.