This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77417 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/4000.
    WB: Preliminary experiments in Human Brain (Amygdala, Cerebellum, Cerebral Cortex, Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). Predicted molecular weight: 46 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      MARCH4, an E3 ubiquitin protein ligase, may mediate ubiquitination of MHC I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin protein ligases accept ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
    • Cellular localization
      Golgi apparatus membrane; Multipass membrane protein.
    • Database links
    • Alternative names
      • BC056494 antibody
      • E3 ubiquitin protein ligase MARCH4 antibody
      • KIAA1399 antibody
      • MARCH 4 antibody
      • MARCH IV antibody
      • Membrane associated RING CH protein IV antibody
      • Membrane associated ring finger (C3HC4) 4 antibody
      • Membrane associated RING finger protein 4 antibody
      • MGC104908 antibody
      • mKIAA1399 antibody
      • RING finger protein 174 antibody
      • RNF174 antibody
      see all

    References

    ab77417 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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