This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77417 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

  • Application notesPeptide ELISA: Antibody detection limit dilution 1/4000.
    WB: Preliminary experiments in Human Brain (Amygdala, Cerebellum, Cerebral Cortex, Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). Predicted molecular weight: 46 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMARCH4, an E3 ubiquitin protein ligase, may mediate ubiquitination of MHC I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin protein ligases accept ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
    • Cellular localizationGolgi apparatus membrane; Multipass membrane protein.
    • Database links
    • Alternative names
      • BC056494 antibody
      • E3 ubiquitin protein ligase MARCH4 antibody
      • KIAA1399 antibody
      • MARCH 4 antibody
      • MARCH IV antibody
      • Membrane associated RING CH protein IV antibody
      • Membrane associated ring finger (C3HC4) 4 antibody
      • Membrane associated RING finger protein 4 antibody
      • MGC104908 antibody
      • mKIAA1399 antibody
      • RING finger protein 174 antibody
      • RNF174 antibody
      see all

    References for Anti-MARCH4 antibody (ab77417)

    ab77417 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77417.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"