Overview

  • Product name
  • Description
    Rabbit polyclonal to MARCH5
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Non human primates, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human MARCH5.

    (Peptide available as ab87426.)

  • Positive control
    • This antibody gave a positive signal in Human Heart Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).

Target

  • Function
    Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
  • Tissue specificity
    Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-CH-type zinc finger.
  • Domain
    The RING-CH-type zinc finger domain is required for E3 ligase activity.
  • Post-translational
    modifications
    Autoubiquitinated leading to degradation (short half-life).
  • Cellular localization
    Mitochondrion outer membrane. Endoplasmic reticulum membrane. Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase MARCH5 antibody
    • FLJ20445 antibody
    • MARCH-V antibody
    • MARCH5 antibody
    • MARH5_HUMAN antibody
    • Membrane-associated ring finger (C3HC4) 5 antibody
    • Membrane-associated RING finger protein 5 antibody
    • Membrane-associated RING-CH protein V antibody
    • Mitochondrial ubiquitin ligase antibody
    • MITOL antibody
    • RING finger protein 153 antibody
    • RNF153 antibody
    see all

Images

  • Anti-MARCH5 antibody (ab77585) at 1 µg/ml + Heart (Human) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab77585 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77585, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Tabony AM  et al. Protein phosphatase 2C-alpha knockdown reduces angiotensin II-mediated skeletal muscle wasting via restoration of mitochondrial recycling and function. Skelet Muscle 4:20 (2014). Read more (PubMed: 25625009) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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