Overview

  • Product nameAnti-MARCH6 antibody
    See all MARCH6 primary antibodies
  • Description
    Goat polyclonal to MARCH6
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide:

    QGRRVIFQKVKE

    , corresponding to amino acids 701-712 of human MARCH6 (NP_005876.2)

  • Positive control
    • Human lung lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16000.
    WB: Use at a concentration of 0.2 - 0.5 µg/ml. Predicted molecular weight: 103 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMARCH6 (Membrane associated RING finger protein 6) belongs to the MARCH family, which contains at least seven membrane associated RING-CH (MARCH)proteins. MARCH proteins are E3 ubiquitin ligases and are located to subcellular membranes.
    • Cellular localizationEndoplasmic reticulum membrane; Multi pass membrane protein.
    • Database links
    • Alternative names
      • Doa10 homolog antibody
      • E3 ubiquitin protein ligase MARCH6 antibody
      • KIAA0597 antibody
      • MARCH 6 antibody
      • MARCH VI antibody
      • MARCHVI antibody
      • Membrane associated RING CH protein VI antibody
      • Membrane associated ring finger (C3HC4) 6 antibody
      • Membrane associated RING finger protein 6 antibody
      • Protein TEB 4 antibody
      • Protein TEB4 antibody
      • RING finger protein 176 antibody
      • RNF 176 antibody
      • RNF176 antibody
      • TEB 4 antibody
      • TEB4 antibody
      see all

    Anti-MARCH6 antibody images

    • Anti-MARCH6 antibody (ab77599) at 0.2 µg/ml + human lung lysate in RIPA buffer at 35 µg

      Predicted band size : 103 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 18 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-MARCH6 antibody (ab77599)

    ab77599 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77599.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"