Overview

  • Product name
  • Description
    Goat polyclonal to MARCH6
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide:

    QGRRVIFQKVKE

    , corresponding to amino acids 701-712 of human MARCH6 (NP_005876.2)

  • Positive control
    • Human lung lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16000.
    WB: Use at a concentration of 0.2 - 0.5 µg/ml. Predicted molecular weight: 103 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      MARCH6 (Membrane associated RING finger protein 6) belongs to the MARCH family, which contains at least seven membrane associated RING-CH (MARCH)proteins. MARCH proteins are E3 ubiquitin ligases and are located to subcellular membranes.
    • Cellular localization
      Endoplasmic reticulum membrane; Multi pass membrane protein.
    • Database links
    • Alternative names
      • DOA10 antibody
      • Doa10 homolog antibody
      • E3 ubiquitin protein ligase MARCH6 antibody
      • KIAA0597 antibody
      • MARCH 6 antibody
      • MARCH VI antibody
      • MARCHVI antibody
      • Membrane associated RING CH protein VI antibody
      • Membrane associated ring finger (C3HC4) 6 antibody
      • Membrane associated RING finger protein 6 antibody
      • Protein TEB 4 antibody
      • Protein TEB4 antibody
      • RING finger protein 176 antibody
      • RNF 176 antibody
      • RNF176 antibody
      • TEB 4 antibody
      • TEB4 antibody
      see all

    Images

    • Anti-MARCH6 antibody (ab77599) at 0.2 µg/ml + human lung lysate in RIPA buffer at 35 µg

      Predicted band size : 103 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 18 kDa. We are unsure as to the identity of these extra bands.

    References

    ab77599 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77599.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up