Overview

  • Product name
  • Description
    Rabbit polyclonal to MARCH8
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A 17 amino acid synthetic peptide from near the C-terminus of Human MARCH8.

  • Positive control
    • HeLa cell lysate; Human Placenta and Human Prostate tissue; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab109690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 33 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC Use a concentration of 2.5 µg/ml.

Target

  • Function
    E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.
  • Tissue specificity
    Broadly expressed. Present in immature dendritic cells (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-CH-type zinc finger.
  • Domain
    The RING-CH-type zinc finger domain is required for E3 ligase activity.
  • Cellular localization
    Cytoplasmic vesicle membrane. Lysosome membrane. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c MIR antibody
    • c-MIR antibody
    • Cellular modulator of immune recognition antibody
    • E3 ubiquitin protein ligase MARCH8 antibody
    • E3 ubiquitin-protein ligase MARCH8 antibody
    • MARCH 8 antibody
    • MARCH VIII antibody
    • MARCH-VIII antibody
    • MARCH8 antibody
    • MARH8_HUMAN antibody
    • Membrane associated RING CH protein VIII antibody
    • Membrane associated ring finger (C3HC4) 8 antibody
    • Membrane associated RING finger protein 8 antibody
    • Membrane-associated RING finger protein 8 antibody
    • Membrane-associated RING-CH protein VIII antibody
    • MIR antibody
    • OTTHUMP00000058921 antibody
    • RING finger protein 178 antibody
    • RNF178 antibody
    see all

Images

  • Lane 1 : Anti-MARCH8 antibody (ab109690) at 0.5 µg/ml
    Lane 2 : Anti-MARCH8 antibody (ab109690) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 33 kDa
  • ab109690, at 5 µg/ml, staining MARCH8 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.
  • ab109690, at 5 µg/ml, staining MARCH8 in formalin-fixed, paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • ab109690, at 2.5 µg/ml, staining MARCH8 in HeLa cells Immunocytochemistry.

References

ab109690 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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