The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.
Broadly expressed. Present in immature dendritic cells (at protein level).
Protein modification; protein ubiquitination.
Contains 1 RING-CH-type zinc finger.
The RING-CH-type zinc finger domain is required for E3 ligase activity.
Cytoplasmic vesicle membrane. Lysosome membrane. Early endosome membrane.