Overview

  • Product name
  • Description
    Rabbit polyclonal to MARCH8
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MSMPLHQISA IPSQDAISAR VYRSKTKEKE REEQNEKTLG HFMSHSSNIS) of Human MARCH8 (NP_001002265).

  • Positive control
    • PANC1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.
  • Tissue specificity
    Broadly expressed. Present in immature dendritic cells (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-CH-type zinc finger.
  • Domain
    The RING-CH-type zinc finger domain is required for E3 ligase activity.
  • Cellular localization
    Cytoplasmic vesicle membrane. Lysosome membrane. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c MIR antibody
    • c-MIR antibody
    • Cellular modulator of immune recognition antibody
    • E3 ubiquitin protein ligase MARCH8 antibody
    • E3 ubiquitin-protein ligase MARCH8 antibody
    • MARCH 8 antibody
    • MARCH VIII antibody
    • MARCH-VIII antibody
    • MARCH8 antibody
    • MARH8_HUMAN antibody
    • Membrane associated RING CH protein VIII antibody
    • Membrane associated ring finger (C3HC4) 8 antibody
    • Membrane associated RING finger protein 8 antibody
    • Membrane-associated RING finger protein 8 antibody
    • Membrane-associated RING-CH protein VIII antibody
    • MIR antibody
    • OTTHUMP00000058921 antibody
    • RING finger protein 178 antibody
    • RNF178 antibody
    see all

Images

  • Anti-MARCH8 antibody (ab99229) at 1 µg/ml + PANC1 cell lysate at 10 µg

    Predicted band size : 33 kDa

References

ab99229 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab99229.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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