The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceMARCH9 (membrane-associated RING-CH protein IX) belongs to MARCH family, which contains at least seven membrane-associated RING-CH (MARCH)proteins. All MARCH proteins are ubiquitin ligases and located to subcellular membranes, and several MARCH proteins reduce surface levels of known substrates of the viral K3 family. Two closely related proteins, MARCH-IV and MARCH-IX, reduce surface expression of MHC-I molecules. In the presence of MARCH-IV or MARCH-IX, MHC-I are ubiquitinated and rapidly internalized by endocytosis.
The functional similarity of the MARCH family and the K3 family of viral immune evasion proteins suggests that the viral immune evasion proteins were derived from MARCH proteins, a novel family of transmembrane ubiquitin ligases that seems to target glycoproteins for lysosomal destruction via ubiquitination of the cytoplasmic tail.