Overview

  • Product nameAnti-MARCKS antibody
    See all MARCKS primary antibodies
  • Description
    Mouse monoclonal to MARCKS
  • Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: GAQFSKTAAK GEAAAERPGE AAVASSPSKA NGQENGHVKV NGDASPAAAE SGAKEELQAN GSAP, corresponding to amino acids 2-66 of Human MARCKS

Properties

Applications

Our Abpromise guarantee covers the use of ab55451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • Sequence similaritiesBelongs to the MARCKS family.
  • Post-translational
    modifications
    Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
  • Cellular localizationCytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 80 kDa protein antibody
    • 80K L antibody
    • 80K L protein antibody
    • 80K-L protein antibody
    • 80KL antibody
    • 81 kDa protein, light chain antibody
    • light chain antibody
    • MACS antibody
    • MARCKS antibody
    • MARCS antibody
    • MARCS_HUMAN antibody
    • MGC52672 antibody
    • myristoylated alanine rich C kinase substrate antibody
    • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
    • Myristoylated alanine rich protein kinase C substrate antibody
    • Myristoylated alanine-rich C-kinase substrate antibody
    • Phosphomyristin antibody
    • PKCSL antibody
    • PRKCSL antibody
    • protein kinase C substrate 80 kDa protein light chain antibody
    • Protein kinase C substrate antibody
    see all

Anti-MARCKS antibody images



  • Predicted band size : 31 kDa
    MARCKS antibody (ab55451) at 1ug/lane + Raw cell lysate at 25ug/lane.
  • ab55451 at 10 ug/ml staining MARCKS in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab55451 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55451, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-MARCKS antibody (ab55451)

ab55451 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab55451.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"