Overview

  • Product nameAnti-MARCKS antibody
    See all MARCKS primary antibodies
  • Description
    Rabbit polyclonal to MARCKS
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
    Predicted to work with: all Mammals
  • Immunogen

    Recombinant full length rat MARCKS expressed in and purified from E. coli.

  • Positive control
    • rat cerebral cortex lysate, Mixed neuron/glial cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab72459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 30 kDa).
ICC/IF 1/500.

Target

  • FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • Sequence similaritiesBelongs to the MARCKS family.
  • Post-translational
    modifications
    Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
  • Cellular localizationCytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 80 kDa protein antibody
    • 80K L antibody
    • 80K L protein antibody
    • 80K-L protein antibody
    • 80KL antibody
    • 81 kDa protein, light chain antibody
    • light chain antibody
    • MACS antibody
    • MARCKS antibody
    • MARCS antibody
    • MARCS_HUMAN antibody
    • MGC52672 antibody
    • myristoylated alanine rich C kinase substrate antibody
    • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
    • Myristoylated alanine rich protein kinase C substrate antibody
    • Myristoylated alanine-rich C-kinase substrate antibody
    • Phosphomyristin antibody
    • PKCSL antibody
    • PRKCSL antibody
    • protein kinase C substrate 80 kDa protein light chain antibody
    • Protein kinase C substrate antibody
    see all

Anti-MARCKS antibody images

  • Anti-MARCKS antibody (ab72459) at 1/10000 dilution + rat cerebral cortex lysate

    Predicted band size : 30 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Mixed neuron/glial cultures stained with MARCKS antibody ab72459 at 1/500 (red), and with an anti-MAP2 antibody (green).

References for Anti-MARCKS antibody (ab72459)

ab72459 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Oligodendrocyte)
Loading amount 30 µg
Specification Oligodendrocyte
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 27°C
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Verified customer

Submitted May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Oligodendrocyte)
Specification Oligodendrocyte
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted May 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"