Overview

  • Product nameAnti-MARCKS antibody
    See all MARCKS primary antibodies
  • Description
    Rabbit polyclonal to MARCKS
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from Human MARCKS around the phosphorylation site of serine 158 (R-F-SP-F-K).

  • Positive control
    • WB: extracts from Mouse brain cells. ICC/IF: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab78885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • Sequence similaritiesBelongs to the MARCKS family.
  • Post-translational
    modifications
    Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
  • Cellular localizationCytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 80 kDa protein antibody
    • 80K L antibody
    • 80K L protein antibody
    • 80K-L protein antibody
    • 80KL antibody
    • 81 kDa protein, light chain antibody
    • light chain antibody
    • MACS antibody
    • MARCKS antibody
    • MARCS antibody
    • MARCS_HUMAN antibody
    • MGC52672 antibody
    • myristoylated alanine rich C kinase substrate antibody
    • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
    • Myristoylated alanine rich protein kinase C substrate antibody
    • Myristoylated alanine-rich C-kinase substrate antibody
    • Phosphomyristin antibody
    • PKCSL antibody
    • PRKCSL antibody
    • protein kinase C substrate 80 kDa protein light chain antibody
    • Protein kinase C substrate antibody
    see all

Anti-MARCKS antibody images

  • All lanes : Anti-MARCKS antibody (ab78885) at 1/500 dilution

    Lane 1 : Extracts from Mouse brain cells
    Lane 2 : Extracts from Mouse brain cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Left: immunofluorescence analysis of A549 cells using ab78885 at 1/500 dilution. Right: same, after pre-treatment with immunizing peptide.

References for Anti-MARCKS antibody (ab78885)

ab78885 has not yet been referenced specifically in any publications.

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These antibodies will bind to the total protein (phospho and non-phospho).

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"