Overview

  • Product name
    Anti-MARCKS antibody [EP1446Y]
    See all MARCKS primary antibodies
  • Description
    Rabbit monoclonal [EP1446Y] to MARCKS
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on human MARCKS.

  • Positive control
    • Fetal brain lysate, human brain tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000. Predicted molecular weight: 32 kDa.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
    • Sequence similarities
      Belongs to the MARCKS family.
    • Post-translational
      modifications
      Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
    • Cellular localization
      Cytoplasm > cytoskeleton. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 80 kDa protein antibody
      • 80K L antibody
      • 80K L protein antibody
      • 80K-L protein antibody
      • 80KL antibody
      • 81 kDa protein, light chain antibody
      • light chain antibody
      • MACS antibody
      • MARCKS antibody
      • MARCS antibody
      • MARCS_HUMAN antibody
      • MGC52672 antibody
      • myristoylated alanine rich C kinase substrate antibody
      • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
      • Myristoylated alanine rich protein kinase C substrate antibody
      • Myristoylated alanine-rich C-kinase substrate antibody
      • Phosphomyristin antibody
      • PKCSL antibody
      • PRKCSL antibody
      • protein kinase C substrate 80 kDa protein light chain antibody
      • Protein kinase C substrate antibody
      see all

    Images

    • ab52616 staining MARCKS in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Control: PBS only.

    • Overlay histogram showing HeLa cells stained with ab52616 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52616, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-MARCKS antibody [EP1446Y] (ab52616) at 1/10000 dilution + Human fetal brain lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab52616 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52616 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Ab52616 at 1/100 dilution staining human brain tissue; paraffin embedded.

    References

    This product has been referenced in:
    • Rohrbach TD  et al. MARCKS phosphorylation is modulated by a peptide mimetic of MARCKS effector domain leading to increased radiation sensitivity in lung cancer cell lines. Oncol Lett 13:1216-1222 (2017). WB ; Human . Read more (PubMed: 28454237) »
    • Dorris E  et al. MARCKS promotes invasion and is associated with biochemical recurrence in prostate cancer. Oncotarget 8:72021-72030 (2017). WB, IHC-P ; Human . Read more (PubMed: 29069765) »

    See all 10 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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