Overview

  • Product nameAnti-MARCKS antibody [EP1446Y]
    See all MARCKS primary antibodies
  • Description
    Rabbit monoclonal [EP1446Y] to MARCKS
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on human MARCKS.

  • Positive control
    • Fetal brain lysate, human brain tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab52616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000. Predicted molecular weight: 32 kDa.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
    • Sequence similaritiesBelongs to the MARCKS family.
    • Post-translational
      modifications
      Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
    • Cellular localizationCytoplasm > cytoskeleton. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 80 kDa protein antibody
      • 80K L antibody
      • 80K L protein antibody
      • 80K-L protein antibody
      • 80KL antibody
      • 81 kDa protein, light chain antibody
      • light chain antibody
      • MACS antibody
      • MARCKS antibody
      • MARCS antibody
      • MARCS_HUMAN antibody
      • MGC52672 antibody
      • myristoylated alanine rich C kinase substrate antibody
      • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
      • Myristoylated alanine rich protein kinase C substrate antibody
      • Myristoylated alanine-rich C-kinase substrate antibody
      • Phosphomyristin antibody
      • PKCSL antibody
      • PRKCSL antibody
      • protein kinase C substrate 80 kDa protein light chain antibody
      • Protein kinase C substrate antibody
      see all

    Anti-MARCKS antibody [EP1446Y] images

    • ab52616 staining MARCKS in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Control: PBS only.

    • Overlay histogram showing HeLa cells stained with ab52616 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52616, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-MARCKS antibody [EP1446Y] (ab52616) at 1/10000 dilution + Human fetal brain lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab52616 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52616 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Ab52616 at 1/100 dilution staining human brain tissue; paraffin embedded.

    References for Anti-MARCKS antibody [EP1446Y] (ab52616)

    This product has been referenced in:
    • Bickeböller M  et al. Functional characterization of the tumor-suppressor MARCKS in colorectal cancer and its association with survival. Oncogene 0:N/A (2014). Human . Read more (PubMed: 24662837) »
    • Chen X  et al. Combined PKC and MEK inhibition in uveal melanoma with GNAQ and GNA11 mutations. Oncogene N/A:N/A (2013). Read more (PubMed: 24141786) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"