Recombinant
RabMAb

Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

Overview

  • Product name
    Anti-MARCKS like protein antibody [EPR15353] - C-terminal
    See all MARCKS like protein primary antibodies
  • Description
    Rabbit Monoclonal [EPR15353] to MARCKS like protein - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MARCKS like protein aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P49006

  • Positive control
    • 293T, SH-SY5Y and Human fetal brain lysates; Human colon tissue; 293T and SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 42-48 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100.
ICC/IF 1/250.
Flow Cyt 1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • All lanes : Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation

  • Immunohistochemical analysis of Paraffin-embedded Human colon tissue labeling MARCKS like protein with ab184546 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/50000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.

  • Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/250 dilution (red) followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/140 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab184546 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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