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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MARCKS like protein aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P49006
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab184546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 42-48 kDa (predicted molecular weight: 20 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation
Immunohistochemical analysis of Paraffin-embedded Human colon tissue labeling MARCKS like protein with ab184546 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.
Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/250 dilution (red) followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/140 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
ab184546 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"