Anti-MARCKS (phospho S152 + S156) antibody (ab32590)

Overview

  • Product nameAnti-MARCKS (phospho S152 + S156) antibody
    See all MARCKS primary antibodies
  • Description
    Rabbit polyclonal to MARCKS (phospho S152 + S156)
  • Specificityab32590 detects Phospho MARCKS (Ser152,156).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152,156 of MARCKS.

  • Positive control
    • Rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab32590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 31 kDa).

Target

  • FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • Sequence similaritiesBelongs to the MARCKS family.
  • Post-translational
    modifications
    Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
  • Cellular localizationCytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 80 kDa protein antibody
    • 80K L antibody
    • 80K L protein antibody
    • 80K-L protein antibody
    • 80KL antibody
    • 81 kDa protein, light chain antibody
    • light chain antibody
    • MACS antibody
    • MARCKS antibody
    • MARCS antibody
    • MARCS_HUMAN antibody
    • MGC52672 antibody
    • myristoylated alanine rich C kinase substrate antibody
    • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
    • Myristoylated alanine rich protein kinase C substrate antibody
    • Myristoylated alanine-rich C-kinase substrate antibody
    • Phosphomyristin antibody
    • PKCSL antibody
    • PRKCSL antibody
    • protein kinase C substrate 80 kDa protein light chain antibody
    • Protein kinase C substrate antibody
    see all

Anti-MARCKS (phospho S152 + S156) antibody images

  • All lanes : Anti-MARCKS (phospho S152 + S156) antibody (ab32590) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat brain lysate with Ser152/156 phosphopeptide used as antigen

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 31 kDa
    Observed band size : 87 kDa (why is the actual band size different from the predicted?)

References for Anti-MARCKS (phospho S152 + S156) antibody (ab32590)

ab32590 has not yet been referenced specifically in any publications.

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The concentration of the anti-MARCKS (phospho S152 + S156) antibody (ab32590) varies slightly on a lot-to-lot basis as this antibody is titered for reproducibility before being put...

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