Overview

  • Product name
    Anti-MARCKS (phospho S158) antibody [EP2113Y]
    See all MARCKS primary antibodies
  • Description
    Rabbit monoclonal [EP2113Y] to MARCKS (phospho S158)
  • Specificity
    This antibody only detects MARCKS phosphorylated at serine 158.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MARCKS.

  • Positive control
    • Hela cell lysates treated with TPA.
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab81295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
    • Sequence similarities
      Belongs to the MARCKS family.
    • Post-translational
      modifications
      Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
    • Cellular localization
      Cytoplasm > cytoskeleton. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 80 kDa protein antibody
      • 80K L antibody
      • 80K L protein antibody
      • 80K-L protein antibody
      • 80KL antibody
      • 81 kDa protein, light chain antibody
      • light chain antibody
      • MACS antibody
      • MARCKS antibody
      • MARCS antibody
      • MARCS_HUMAN antibody
      • MGC52672 antibody
      • myristoylated alanine rich C kinase substrate antibody
      • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
      • Myristoylated alanine rich protein kinase C substrate antibody
      • Myristoylated alanine-rich C-kinase substrate antibody
      • Phosphomyristin antibody
      • PKCSL antibody
      • PRKCSL antibody
      • protein kinase C substrate 80 kDa protein light chain antibody
      • Protein kinase C substrate antibody
      see all

    Anti-MARCKS (phospho S158) antibody [EP2113Y] images

    • All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (purified)

      Lane 1 : Rat brain
      Lane 2 : Mouse brain

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/10000 dilution (purified)

      Lane 1 : HEK293 cells treated with TPA
      Lane 2 : HEK293 cells treated with TPA, and the PVDF membrane treated with lambda phosphatase 10ug

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (unpurified)

      Lane 1 : Hela cell lysates, untreated.
      Lane 2 : Hela cell lysates treated with TPA.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)

    References for Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)

    This product has been referenced in:
    • Rohrbach TD  et al. MARCKS phosphorylation is modulated by a peptide mimetic of MARCKS effector domain leading to increased radiation sensitivity in lung cancer cell lines. Oncol Lett 13:1216-1222 (2017). WB ; Human . Read more (PubMed: 28454237) »
    • Rohrbach TD  et al. Targeting the effector domain of the myristoylated alanine rich C-kinase substrate enhances lung cancer radiation sensitivity. Int J Oncol 46:1079-88 (2015). Read more (PubMed: 25524703) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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