Overview

  • Product nameAnti-MARCKS (phospho S158) antibody [EP2113Y]
    See all MARCKS primary antibodies
  • Description
    Rabbit monoclonal [EP2113Y] to MARCKS (phospho S158)
  • SpecificityThis antibody only detects MARCKS phosphorylated at serine 158.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MARCKS.

  • Positive control
    • Hela cell lysates treated with TPA.
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab81295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
    • Sequence similaritiesBelongs to the MARCKS family.
    • Post-translational
      modifications
      Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
    • Cellular localizationCytoplasm > cytoskeleton. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 80 kDa protein antibody
      • 80K L antibody
      • 80K L protein antibody
      • 80K-L protein antibody
      • 80KL antibody
      • 81 kDa protein, light chain antibody
      • light chain antibody
      • MACS antibody
      • MARCKS antibody
      • MARCS antibody
      • MARCS_HUMAN antibody
      • MGC52672 antibody
      • myristoylated alanine rich C kinase substrate antibody
      • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
      • Myristoylated alanine rich protein kinase C substrate antibody
      • Myristoylated alanine-rich C-kinase substrate antibody
      • Phosphomyristin antibody
      • PKCSL antibody
      • PRKCSL antibody
      • protein kinase C substrate 80 kDa protein light chain antibody
      • Protein kinase C substrate antibody
      see all

    Anti-MARCKS (phospho S158) antibody [EP2113Y] images

    • All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (purified)

      Lane 1 : Rat brain
      Lane 2 : Mouse brain

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/10000 dilution (purified)

      Lane 1 : HEK293 cells treated with TPA
      Lane 2 : HEK293 cells treated with TPA, and the PVDF membrane treated with lambda phosphatase 10ug

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (unpurified)

      Lane 1 : Hela cell lysates, untreated.
      Lane 2 : Hela cell lysates treated with TPA.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)

    References for Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)

    This product has been referenced in:
    • Jarboe JS  et al. MARCKS regulates growth and radiation sensitivity and is a novel prognostic factor for glioma. Clin Cancer Res 18:3030-41 (2012). WB . Read more (PubMed: 22619307) »
    • Rumsby M  et al. Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C. Br J Cancer 104:673-84 (2011). WB ; Human . Read more (PubMed: 21266973) »

    See all 4 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab81295.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"