Overview

  • Product nameAnti-MARK 1+2+3+4 antibodySee all MARK 1+2+3+4 primary antibodies ...
  • Description
    Rabbit polyclonal to MARK 1+2+3+4
  • Specificityab74131 detects endogenous levels of total MARK 1+2+3+4 protein.
  • Tested applicationsWB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human MARK1/2/3/4 around the phosphorylation site of threonine 215 (L-D-TP-F-C).

  • Positive control
    • Human breast carcinoma tissue. Extracts from COS-7 cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 89 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

Anti-MARK 1+2+3+4 antibody images

  • All lanes : Anti-MARK 1+2+3+4 antibody (ab74131) at 1/500 dilution

    Lane 1 : Extracts from COS-7 cells
    Lane 2 : Extracts from COS-7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 89 kDa
    Observed band size : 89 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab74131, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • Immunofluorescence analysis of HeLa cells, using ab74131 at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-MARK 1+2+3+4 antibody (ab74131)

This product has been referenced in:
  • Mian I  et al. LKB1 destabilizes microtubules in myoblasts and contributes to myoblast differentiation. PLoS One 7:e31583 (2012). WB ; Mouse . Read more (PubMed: 22348111) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab74131.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"