Overview

  • Product name
  • Description
    Rabbit polyclonal to MARK1
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the N terminal region (human), conjugated to KLH.

  • Positive control
    • IHC-P: Human brain tissue. WB: P7 mouse whole brain lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab60202 was purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab60202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ELISA 1/1000.
WB 1/200 - 1/500. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).

Target

  • Function
    May play a role in cytoskeletal stability.
  • Tissue specificity
    Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Appears to localize to an intracellular network.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1477 antibody
    • MAP/microtubule affinity regulating kinase 1 antibody
    • MAP/microtubule affinity-regulating kinase 1 antibody
    • MARK 1 antibody
    • MARK antibody
    • Mark1 antibody
    • MARK1_HUMAN antibody
    • MGC126512 antibody
    • MGC126513 antibody
    • Serine/threonine protein kinase MARK1 antibody
    • Serine/threonine-protein kinase MARK1 antibody
    see all

Images

  • Anti-MARK1 antibody (ab60202) at 1/250 dilution + P7 mouse whole brain lysate at 60 µg

    Predicted band size : 89 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  • ab60202, at 1/50 dilution, staining MARK1 in formalin fixed, paraffin-embedded human brain tissue by immunohistochemistry. ab60202 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References

ab60202 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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