Overview

  • Product name
    Anti-MARK1 antibody - N-terminal
    See all MARK1 primary antibodies
  • Description
    Rabbit polyclonal to MARK1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within N terminal amino acids 1-265 of Human MARK1 (Uniprot ID Q9P0L2)

  • Positive control
    • A431 whole cell lysate, H1299 whole cell lysate, HeLa whole cell lysate and HeLa paraformaldehyde-fixed cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 89 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May play a role in cytoskeletal stability.
  • Tissue specificity
    Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Appears to localize to an intracellular network.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1477 antibody
    • MAP/microtubule affinity regulating kinase 1 antibody
    • MAP/microtubule affinity-regulating kinase 1 antibody
    • MARK 1 antibody
    • MARK antibody
    • Mark1 antibody
    • MARK1_HUMAN antibody
    • MGC126512 antibody
    • MGC126513 antibody
    • Serine/threonine protein kinase MARK1 antibody
    • Serine/threonine-protein kinase MARK1 antibody
    see all

Images

  • Anti-MARK1 antibody - N-terminal (ab154357) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 89 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MARK1 with ab154357 at 1/200 dilution (green). Bottom panel shows image merged with DNA probe (blue).

References

ab154357 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab154357.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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