For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Rabbit monoclonal [EPR5463] to MARK1 (phospho T215)
WBmore detailsUnsuitable for:
Flow Cyt,ICC/IF,IHC-P or IP
Mouse, Rat, Human
Synthetic phosphopeptide corresponding to residues surrounding Threonine 215 of Human MARK1 (UniProt Q9P0L2).
- SH-SY5Y cell lysates, treated with Okadaic acid and Calyculin A.
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa).
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
May play a role in cytoskeletal stability.
Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Cytoplasm > cytoskeleton. Appears to localize to an intracellular network.
Information by UniProt
- KIAA1477 antibody
- MAP/microtubule affinity regulating kinase 1 antibody
- MAP/microtubule affinity-regulating kinase 1 antibody
Western blot - Anti-MARK1 (phospho T215) antibody [EPR5463] (ab126731)
All lanes : Anti-MARK1 (phospho T215) antibody [EPR5463] (ab126731) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate, untreated
Lane 2 : SH-SY5Y cell lysate, treated with Okadaic acid and
Lysates/proteins at 10 µg per lane.
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 89 kDa
Other-Anti-MARK1 (phospho T215) antibody [EPR5463](ab126731)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"