Recombinant
RabMAb

Anti-MARK1 (phospho T215) antibody [EPR5463] (ab126731)

Overview

  • Product name
    Anti-MARK1 (phospho T215) antibody [EPR5463]
    See all MARK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5463] to MARK1 (phospho T215)
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Threonine 215 of Human MARK1 (UniProt Q9P0L2).

  • Positive control
    • SH-SY5Y cell lysates, treated with Okadaic acid and Calyculin A.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      May play a role in cytoskeletal stability.
    • Tissue specificity
      Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Cellular localization
      Cytoplasm > cytoskeleton. Appears to localize to an intracellular network.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1477 antibody
      • MAP/microtubule affinity regulating kinase 1 antibody
      • MAP/microtubule affinity-regulating kinase 1 antibody
      • MARK 1 antibody
      • MARK antibody
      • Mark1 antibody
      • MARK1_HUMAN antibody
      • MGC126512 antibody
      • MGC126513 antibody
      • Serine/threonine protein kinase MARK1 antibody
      • Serine/threonine-protein kinase MARK1 antibody
      see all

    Images

    • All lanes : Anti-MARK1 (phospho T215) antibody [EPR5463] (ab126731) at 1/10000 dilution

      Lane 1 : SH-SY5Y cell lysate, untreated
      Lane 2 : SH-SY5Y cell lysate, treated with Okadaic acid and Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 89 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126731 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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