• Product nameAnti-Mark3 antibody
    See all Mark3 primary antibodies
  • Description
    Mouse monoclonal to Mark3
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Chimpanzee
  • Immunogen

    Recombinant fragment: HHKVQRSVFS SQKQRRYSDH AGPAIPSVVA YPKRSQTSTA DSDLKEDGIS SRKSSGSAVG GKGIAPASPM LGNASNPNKA DIPERKKSST VPSSNTASG, corresponding to amino acids 402-500 of Human Mark3 (NP_002367) with a 26 kDa tag.

  • Positive control
    • Mark3 transfected 293T cell lysate. Recombinant tagged human Mark3 fragment.



Our Abpromise guarantee covers the use of ab77199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 87 kDa.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 0.01-0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged Mark3 is approximately 3ng/ml when used as a capture antibody.


  • FunctionInvolved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-TAK1 antibody
    • Cdc25C associated protein kinase 1 antibody
    • Cdc25C-associated protein kinase 1 antibody
    • cTAK1 antibody
    • ELKL motif kinase 2 antibody
    • EMK-2 antibody
    • Emk2 antibody
    • ETK 1 antibody
    • KIAA4230 antibody
    • KP78 antibody
    • MAP microtubule affinity regulating kinase 3 antibody
    • MAP/microtubule affinity-regulating kinase 3 antibody
    • Mark3 antibody
    • MARK3_HUMAN antibody
    • Par 1a antibody
    • PAR1A antibody
    • Protein kinase STK10 antibody
    • Ser/Thr protein kinase PAR-1 antibody
    • Serine threonine protein kinase p78 antibody
    • Serine/threonine-protein kinase p78 antibody
    • SerThr protein kinase PAR 1 antibody
    see all

Anti-Mark3 antibody images

  • Overlay histogram showing HeLa cells stained with ab77199 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77199, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-Mark3 antibody (ab77199) at 5 µg/ml + Recombinant tagged human Mark3 fragment at 0.2 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 87 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Western blot against the tagged recombinant protein immunogen, which has a predicted molecular weight of 37 kDa.
  • All lanes : Anti-Mark3 antibody (ab77199) at 5 µg/ml

    Lane 1 : Mark3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 87 kDa
    Observed band size : 87 kDa
  • Detection limit for ab77199 is approximately 3ng/ml as a capture antibody.
  • ICC/IF image of ab77199 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77199, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Mark3 antibody (ab77199)

ab77199 has not yet been referenced specifically in any publications.

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