Overview

  • Product nameAnti-MARK4 antibody
    See all MARK4 primary antibodies
  • Description
    Rabbit polyclonal to MARK4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 605-633 of Mouse MARK4 (NP_758483.1).

  • Positive control
    • Mouse testis tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab124228 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 82 kDa.

Target

  • FunctionSerine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).
  • Tissue specificityUbiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Developmental stageExpressed at all stages of the mitotic cell cycle.
  • Post-translational
    modifications
    Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
    Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).
  • Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ90097 antibody
    • KIAA1860 antibody
    • MAP / microtubule affinity regulating kinase 4 antibody
    • MAP / microtubule affinity regulating kinase 4L antibody
    • MAP/microtubule affinity regulating kinase 4 antibody
    • MAP/microtubule affinity regulating kinase like 1 antibody
    • MAP/microtubule affinity-regulating kinase 4 antibody
    • MAP/microtubule affinity-regulating kinase-like 1 antibody
    • MARK 4 antibody
    • MARK 4L antibody
    • MARK L1 antibody
    • MARK4 antibody
    • MARK4 serine / threonine protein kinase antibody
    • MARK4 serine/threonine protein kinase antibody
    • MARK4_HUMAN antibody
    • MARK4S antibody
    • MARKL 1 antibody
    • MARKL1 antibody
    • Nbla00650 antibody
    • Putative protein product of Nbla00650 antibody
    see all

Anti-MARK4 antibody images

  • Anti-MARK4 antibody (ab124228) at 1/100 dilution + Mouse testis tissue lysate at 35 µg

    Predicted band size : 82 kDa

References for Anti-MARK4 antibody (ab124228)

This product has been referenced in:
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). WB ; Mouse . Read more (PubMed: 26888669) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"