The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 19 kDa.
Use a concentration of 5 µg/ml.
FunctionMicrotubule-associated protein that exhibits cell cycle-dependent localization and can inhibit cell proliferation and migration.
Tissue specificityWidely expressed in normal tissues. Down-regulated in multiple primary tumors.
Sequence similaritiesContains 1 MARVEL domain.
Cellular localizationCell membrane. Cytoplasm > cytoskeleton. Nucleus. Observed in the nucleus and at the perinuclear region during interphase, but localizes at the mitotic spindle and midbody at metaphase. A significant fraction of MARVELD1 translocates to the plasma membrane during anaphase or upon microtubule depolymerization.
ICC/IF image of ab91640 stained PC3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91640 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - MARVELD1 antibody (ab91640)
Lane 1 : Anti-MARVELD1 antibody (ab91640) at 1 µg/ml Lane 2 : Anti-MARVELD1 antibody (ab91640) at 2 µg/ml
Lane 1 : Mouse heart lysate Lane 2 : Mouse heart lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 19 kDa
References for Anti-MARVELD1 antibody (ab91640)
This product has been referenced in:
Hu J et al. MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1. PLoS One8:e68291 (2013).
Read more (PubMed: 23826386) »